Multiplex immunofluorescence biomarker imaging allows for multi-label analysis of formalin-fixed paraffin embedded (FFPE) tissue biopsy samples, enabling the study of complex spatial biology within the tumor and the tumor microenvironment.
We use Akoya’s multiplex biomarker imaging systems to quantify multiple fluorescent molecular markers simultaneously, even when co-localized within a single tissue section. The data for each marker is spectrally unmixed, generating clear and accurate images for each of the individual labels in a multi-labeled tissue section with minimal bleedthrough.
Among the advantages of this quantitative pathology imaging system are the preservation of the morphological context down to the subcellular level, elimination of auto-fluorescence, capturing of low signal levels, and excellent overall quantification of protein expression.
Our laboratory offers help and expertise in the following applications:
- Optimization of novel antibodies
- Developing custom multiplex immunofluorescent panels
- Automated immunostaining
- Image acquisition
- Data and image analysis
We are happy to customize multiplex immunofluorescence panels upon request!