Based on an assay that was developed for Hodgkin lymphoma in Dr. Margaret Shipp's laboratory, we perform FISH on tissue sections to assess copy number on chromosome 9p24.1.
We use bacterial artificial chromosome probes, which maps to 9p24.1 and includes CD274 (encoding PD-L1, labeled with Spectrum Orange), and RP11-635N21, which also maps to 9p24.1 and includes PDCD1LG2 (encoding PD-L2, labeled with Spectrum Green). In addition, we also use a control centromeric probe, Spectrum Aqua–labeled CEP9 (Abbott Molecular) that maps to 9p11-q11.
Our laboratory offers help and expertise in the following applications:
- Optimization of novel FISH probes
- Application and data acquisition
- Data analysis
